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KMID : 1149220130210020081
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Korean Journal of Oriental Medical Prescription
2013 Volume.21 No. 2 p.81 ~ p.89
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Effect of Coptidis Rhizoma Extract on Cytokine Production of Mouse Macrophages
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Kim Bok-Kee
Lee Young-Jong
Han Hyo-Sang
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Abstract
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Objectives : The purpose of this study is to observe the effect of Coptidis Rhizoma (CCE-extract of C. chinensis Rhizome) in induction of immune protein on mouse macrophages.
Methods : To analyze cytokines interleukin(IL)-1α, IL-3, IL-9, IL-12p40, IL-13, IL-17, Monocyte Chemoattractant Protein(MCP)-1 induced by macrophages, mouse macrophages were incubated with CCE and was measured.
Results : IL-1α measurement, CCE showed significant inhibition only at concentration level of 200 ㎍/mL. IL-3, MCP-1 measurement, CCE showed significant inhibition only at concentration level of 100, 200 ㎍/mL. IL-9 measurement, CCE showed significant inhibition only at concentration level of 50 ㎍/mL. IL-13 measurement, CCE showed significant inhibition only at concentration level of 50, 100, 200 ㎍/mL. The IL-12p40, IL-17 levels indicated no changes at 25, 50, 100, 200 ㎍/mL on mouse macrophages.
Conclusions : CCE did not significantly increased inflammatory cytokines IL-1α, IL-3, IL-9, IL-12p40, IL-13, IL-17, Monocyte Chemoattractant Protein(MCP)-1 on mouse macrophages. It was verified CCE does not trigger cytokine related hypersensitivity reaction of organism or exacerbation of acute/chronic inflammatory disease.
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KEYWORD
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Coptidis Rhizoma, Macrophage, Cyokine, Interleukin-1α, Interleukin-17
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